Journal: STAR Protocols

Article Title: CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells

doi: 10.1016/j.xpro.2022.101269

Figure Lengend Snippet:

Article Snippet: Maxpar nuclear antigen staining buffer set , Fluidigm , Cat#201063.

Techniques: Recombinant, Saline, Staining, Software, Cytometry

Journal: Nanomaterials

Article Title: Comparison of Metal-Based Nanoparticles and Nanowires: Solubility, Reactivity, Bioavailability and Cellular Toxicity

doi: 10.3390/nano12010147

Figure Lengend Snippet: FRAS testing ( A ) Cu NP, Cu NP ions, Cu NW and Cu NW ions ( B ) Ni NP, Ni NP ions, Ni NW and Ni NW ions ( C ) TiO 2 NP and TiO 2 NW. Error bars indicate one standard deviation from triplicate testing, and are smaller than the size of the symbol in most cases. Statistics were performed using either ANOVA-Dunnett’s T3 (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001) or the 2-sided Dunnett’s test (‡ p ≤ 0.05, ‡‡‡ p ≤ 0.001).

Article Snippet: The SOP, which described the multi-dose protocol of the Ferric Reduction Ability of Serum (FRAS) assay, published in 2017 by BASF [ ], was used for reactivity testing of samples under physiological conditions.

Techniques: Standard Deviation

Journal: International journal of oncology

Article Title: Impact of Helicobacter pylori on the growth of hepatic orthotopic graft tumors in mice.

doi: 10.3892/ijo.2015.3107

Figure Lengend Snippet: Figure 6. The (A-D) PCNA, (E-H) Bcl-2 and (I-L) Bax expression in the livers and tumors of representative sections. The integrated optical density (IOD) of the positive cells was assessed and expressed as mean ± SD (Table II). IHC stain (original magnification, x400). (A, E and I) Liver tissues of H. pylori-infected mice (group A1 and A2). (B, F and J) Liver tissues of uninfected mice (group B1 and B2). (C, G and K) Tumor tissues of H. pylori-infected mice (group A1). (D, H and L) Tumor tissues of uninfected mice (group B1).

Article Snippet: Sections were then incubated with polyclonal rabbit anti-H. pylori antibody (1:50 diluted in PBS, Product no. B047101; Dako, Glostrup, Denmark), monoclonal mouse anti-PCNA antibody (1:200 diluted in PBS, Product no. sc-56; Santa Cruz Biotechnology, Dallas, TX, USA), monoclonal mouse anti-Bcl-2 antibody (1:50 diluted in PBS, Product no. sc-7382; Santa Cruz Biotechnology), or polyclonal rabbit anti-Bax antibody (1:50 diluted in PBS, Product no. sc-526; Santa Cruz Biotechnology) overnight at 4 ̊C.

Techniques: Expressing, Staining, Infection

Journal: International journal of oncology

Article Title: Impact of Helicobacter pylori on the growth of hepatic orthotopic graft tumors in mice.

doi: 10.3892/ijo.2015.3107

Figure Lengend Snippet: Figure 7. Expression of (A) PCNA, (B) Bcl-2 and (C) Bax in the livers and tumors was assessed using western blot analysis. The integrated optical density (IOD) of each antigen band was measured. The results are expressed as mean ± SD in the histogram. Lanes 1 and 2, the protein expression in the livers and tumors, respectively, of the H. pylori-infected and tumor positive mice (group A1). Lane 3, the protein expression in the livers of H. pylori-infected and tumor negative mice (group A2). Lanes 4 and 5, the protein expression in the livers and tumors, respectively, of the uninfected and tumor positive mice (group B1). Lane 6, the protein expression in the livers of uninfected and tumor negative mice (group B2). *P<0.05 as lane 1 compared with lanes 4 or 6, lane 3 compared with lanes 4 or 6, and lane 2 compared with lane 5.

Article Snippet: Sections were then incubated with polyclonal rabbit anti-H. pylori antibody (1:50 diluted in PBS, Product no. B047101; Dako, Glostrup, Denmark), monoclonal mouse anti-PCNA antibody (1:200 diluted in PBS, Product no. sc-56; Santa Cruz Biotechnology, Dallas, TX, USA), monoclonal mouse anti-Bcl-2 antibody (1:50 diluted in PBS, Product no. sc-7382; Santa Cruz Biotechnology), or polyclonal rabbit anti-Bax antibody (1:50 diluted in PBS, Product no. sc-526; Santa Cruz Biotechnology) overnight at 4 ̊C.

Techniques: Expressing, Western Blot, Infection